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To determine no matter whether miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML affected individual samples and three lineage-depleted (Lin–) twine blood (CB) samples making use of CD34 and CD38 into 4 populations and subjected Every single to worldwide miRNA profiling; the stem mobile articles of each and every fraction was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation calculated by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was lessened in two of a few samples on 126KD (Figures 5C and S5D). Jointly, these results propose that 126KD generates heterogeneous responses with LSC operate and frequency diminished in the subset of AML sufferers.
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(E) Percent BrdU incorporation into bulk cultures demonstrating proliferation of CTRL and 126OE transduced 8227 cells over time.
(D) Representative intracellular move plots to the detection of CDK3 and pRB Ser807/811. Graph beneath signifies three impartial intracellular movement experiments for every problem exactly where the necessarily mean fluorescence intensity was compared. Signify ± SEM; ∗p=
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